BlastStation: Products, BlastStation-Free

699 Registered Users Worldwide

BlastStation-Free is the Free Edition of BlastStation.
BlastStation-Free in the Cloud is a web-based 64-bit local BlastStation running on the Cloud computer and has all features of BlastStation-Workgroup with some limitations. You can upload your own FASTA or FASTQ file, create blast database and perform blast searches without installing any software.
BlastStation-Free ver. 2 has all features of BlastStation2, such as NCBI-Blast search using the EBI server, WU-Blast2 search using the EBI server, and Standalone BLAST search on your PC with some limitations. BlastStation-Free ver. 2 is available for Windows and Mac OS X.

Platform

Requirements

How to install

Cloud

Current Version: 1.0

Change history is here

FireFox 4, Google Chrome, Safari 5.0

or later on Windows, Mac OS X,

and Linux

1. Click Sign Up button and create account.

2. Sign In from here

3. Start local blast search.

Features & Limitations

Features

Local blast search with 64-bit NCBI BLAST+ 2.2.25.
Supports megablast.
Easy database creation from your FASTA or FASTQ file, which can be compressed in .gz, .Z, or .zip format.
Easy multi-FASTA data submission.
Easy management of BLAST search parameters, FASTA data and search results.
Graphical display of search results.
Summary table display of search results.
Export summary table in CSV format.
Export search results sequences in FASTA format.

Limitations

Up to 5 FASTA data submission in one BlastStation job. BlastStation-Workgroup can submit unlimited number of FASTA data in one job.
Available programs are megablast, blastn, blastp, and blastx. If tblastn or tblastx is needed, BlastStation-Workgroup is suitable.
Local database input file size is limited to 25 MB, either compressed or not compressed.
BLAST search and database sharing functions are disabled.
No support. Facebook is available instead.
Signing up is required.

Points will be accumulated as the following schedule. Monthly allowance is 30 points.

	Action	Points
	Performing Blast Search/Sequence	1
	Creating Local Database/Action	2
	Exporting FASTA/Action	2
	Others	0

Use behaviors will be monitored by us and will be used for future improvements.

Windows

Current Version: 2.0

Change history is here

Windows XP, Windows Vista, Windows 7

512MB RAM

1. Click Download button.

2. Click "Run" button.

3. Wait until download finishes.

4. Click "Run" button.

5. Follow the instruction of installer.

Features & Limitations

Features

Local blast search with 32-bit NCBI BLAST 2.2.20.
Easy database creation from your FASTA file.
Easy multi-FASTA data submission.
Easy management of BLAST search parameters, FASTA data and search results.
Graphical display of search results.
Summary table display of search results.
Export summary table in CSV format.
Export search results sequences in FASTA format.

Limitations

Up to 5 FASTA data submission in one BlastStation job. BlastStation2, BlastStation-Local, BlastStation-Local64 can submit unlimited number of FASTA data in one job.
Available programs are megablast, blastn, blastp, and blastx. If tblastn or tblastx is needed, BlastStation2 is suitable.
Local database input file size is limited to 25 MB. If larger database is needed to be created, switch to BlastStation2 or BlastStation-Local.
One core is used for local blast search. If high performance blast search with multiple cores is needed, try BlastStation2 or BlastStation-Local64, which utilizes all available cores.
No support. Facebook is available instead.
Signing up is required.

Points will be accumulated as the following schedule. Monthly allowance is 30 points.

	Action	Points
	Performing Blast Search/Sequence	1
	Creating Local Database/Action	2
	Exporting FASTA/Action	2
	Others	0

Use behaviors will be monitored by us and will be used for future improvements.

Mac OS X

Current Version: 2.1

Change history is here

Mac OS X 10.4.11, 10.5.8, 10.6.8, 10.7.4, OS X 10.8.2 or later

Universal Binary

512MB RAM

1. Click Download button.

2. Wait until donwload finishes.

3. Double click BlastStation-Free.pkg, if installer does not start.

4. Follow the instruction of installer.

Signed for OS X 10.8

Features & Limitations

Features

NCBI-Blast search using the EBI server via REST web service.
WU-Blast2 search using the EBI server via REST web service..
Local blast search with 32-bit NCBI BLAST 2.2.25 on your PC.
Easy database download from NCBI ftp server to your PC.
Easy database creation from your FASTA or FASTQ file, which can be compressed in .gz, .Z, or .zip format.
Easy multi-FASTA data submission.
Easy management of BLAST search parameters, FASTA data and search results.
Graphical display of search results.
Summary table display of search results.
Export summary table in CSV format.
Export search results sequences in FASTA format.

Limitations

Up to 5 FASTA data submission in one BlastStation job. BlastStation2, BlastStation-Local, BlastStation-Local64 can submit unlimited number of FASTA data in one job.
Available programs are blastn, blastp, and blastx. If tblastn or tblastx is needed, BlastStation2 is suitable.
Local database input file size is limited to 25 MB, either compressed or not compressed. If larger database is needed to be created, upgrade to the premium account, or switch to BlastStation2 or BlastStation-Local.
One core is used for local blast search. If high performance blast search with multiple cores is needed, try BlastStation2 or BlastStation-Local64 with 64-bit NCBI Blast+, which utilizes all available cores.
Function to change database folder is disabled.
Function to display alignment detail is disabled.
No support. Facebook is available instead.
Signing up is required.

Points will be accumulated as the following schedule. Monthly allowance is 30 points.

	Action	Points
	Performing Blast Search/Sequence	1
	Creating Local Database/Action	2
	Exporting FASTA/Action	2
	Others	0

Use behaviors will be monitored by us and will be used for future improvements.

Premium account is available with nominal fee. Please log in to upgrade.

	Free	Premium
DB input file size limit even compressed	25 MB	250 MB
Support	Facebook	Email Support in Account Page
Allowance	30 points/month	Unlimited as long as Premium status is active
Cost	Free	US$29.99/week nonrecurring charge

¹⁾If you would like to use both of BlastStation-Free in the Cloud and BlastStation-Free ver.2, you need to sign up twice with different email addresses.

²⁾BlastStation-Free ver. 2 installer will overwrite BlastStation-Free ver. 1.2 or earlier, which is not maintained anymore.