BlastStation-Local HelpWelcomeIntroduction to BlastStation-LocalFASTA data inputJob submissionHow to read resultsInstructions in detailDownload databaseCreate databaseRemove databaseChange database folderDelete jobsBackup jobsRestore jobsSearch parameters settingsNavigation in result windowExport search resultsExport FASTA dataPreference settingsData foldersMenu ReferenceBlastStation-Local menuFile menuEdit menuJob menuTools menuHelp menu
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Create databaseYou can create database files from your own multi-FASTA data or FASTQ data. Plain, .gz, .Z, and .zip text files are accepted. FASTA data format is defined here and multi-FASTA data format is defined here. On the Tools menu, click Create DB. The Create DB window opens. Clicking "Select Input File" button will open File Open dialog. Select input file and click "Open" button. Or drag and drop input file.
Several optional parameters can be specified. Usually these options do not have to be used. DB Title: Adds custom title to the database. Indexing:If input FASTA file has NCBI styled deflines, turn it ON. Default value is OFF. It is identical to "-o T" formatdb option. Advanced Options: formatdb options can be directly specified here. Please use this option only if you know what you are doing. Clicking "Create DB" button creates database. When database is created, "Database is created" message will be shown. BlastStation-Local64 Only When selected input file is large, multiple database files such as nt.00, nt.01, and so on will be created. At the same time, the alias file, such as nt.nal will be created. When this alias file is selected as database, Blast+ search over large virtual database can be done.
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