BlastStation-Local Help

Welcome

Introduction to BlastStation-Local

 FASTA data input

 Job submission

 How to read results

Instructions in detail

 Download database

 Create database

 Remove database

 Delete jobs

 Backup jobs

 Restore jobs

 Search parameters settings

 Navigation in result window

 Export search results

 Export FASTA data

 Preference settings

 Data directory

Menu Reference

 File menu

 Edit menu

 Job menu

 Tools menu

 Help menu

 

www.BlastStation.com

 

Create database

You can create database files from your own multi-FASTA data.  FASTA data format is defined here and multi-FASTA data format is defined here.

On the Tools menu, click Create DB.  The Create DB window opens.

Clicking "Select FASTA File" button will open File Open dialog. Select multi-FASTA file and click "Open" button.

Selected FASTA file name is shown in the "DB Name" field.  Change it if necessary.

Several optional parameters can be specified.  Usually these options do not have to be used.

DB Title: Adds custom title to the database.

Indexing:If input FASTA file has NCBI styled deflines, turn it ON.  Default value is OFF.  It is identical to "-o T" formatdb option.

Advanced Options: formatdb options can be directly specified here.  Please use this option only if you know what you are doing.

Clicking "Create DB" button creates database.  When database is created,  "Database is created" message will be shown.

"Cancel" button will close Create DB window.

BlastStation-Local64 Only

When selected FASTA file is large, multiple database files such as nt.00, nt.01, and so on will be created.  At the same time, the alias file, such as nt.nal will be created.  When this alias file is selected as database, Blast search over large virtual database can be done.