BlastStation-FSA Help:How to read results

BlastStation-FSA Help

Introduction to BlastStation-FSA

Instructions in detail

Menu Reference

How to read results

When BLAST search is finished, the following dialog will be displayed.

Let's click "Yes" to see search results.

Bar graph is shown on the upper half. The color of the bar is classified by the identity. Red is 100%, purple is 90% or more, and so on. The length of the query sequence (606 in this case) is given above the black bar at the top. You can easily understand which portion of the subject sequence matches with the query sequence. Move your mouse cursor on the bars and click it. The color of the bar changes and four numbers will be displayed as shown below. The sequence from 19 to 242 of query sequence match with that from 2 to 225 of AY677112.1.

Table is shown on the lower half, which consists of ID, Accession number, Definition, length, bit score, E Value, and Identity.

When you slide the slider (indicated by the red arrow) with your mouse, the next search result is shown.