BlastStation-Free HelpWelcomeIntroduction to BlastStationFASTA data inputJob submissionHow to read resultsRemote BLAST SearchEBI NCBI-BlastEBI WU-Blast2Local BLAST SearchDownload databaseCreate databaseBLAST machine selectionInstructions in detailDelete jobsBackup jobsRestore jobsRemove databaseSearch parameters settingsNavigation in result windowExport search resultsExport FASTA dataPreference settingsData directoryMenu ReferenceBlastStation menuFile menuEdit menuJob menuTools menuHelp menu
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How to read resultsWhen BLAST search is finished, the following dialog will be displayed. Let's click "Yes" to see search results. Bar graph is shown on the upper half. The color of the bar is classified by the identity. Red is 100%, purple is 90% or more, and so on. The length of the query sequence (606 in this case) is given above the black bar at the top. You can easily understand which portion of the subject sequence matches with the query sequence. Move your mouse cursor on the bars. The color of the bar changes and four numbers will be displayed as shown below. The sequence from 19 to 572 of query sequence match with that from 2 to 552 of AY661748. Table is shown on the lower half, which consists of ID, Accession number, Definition, length, bit score, E Value, and Identity. In order to display the other search results, click arrows below.
When you click "Jump" button, the drawer will be shown next to the window. Description of each FASTA file will be listed in this drawer. Clicking each description will display corresponding search result. If you have many descriptions, type in search strings in the text field and hit enter to narrow this list. Clicking "Jump" button again will hide the drawer.
Job > Display Output menu will display Blast results file in the default browser. |