BlastStation-Free Help

Welcome

Introduction to BlastStation

　FASTA data input

　Job submission

　How to read results

Remote BLAST Search

　EBI NCBI-Blast

　EBI WU-Blast2

Local BLAST Search

　Download database

　Create database

　BLAST machine selection

Instructions in detail

　Delete jobs

　Backup jobs

　Restore jobs

　Remove database

　Search parameters settings

　Navigation in result window

　Export search results

　Export FASTA data

　Preference settings

　Data directory

Menu Reference

　BlastStation menu

　File menu

　Edit menu

　Job menu

　Tools menu

　Help menu

www.BlastStation.com

Welcome

BlastStation-Free is a Free Edition of BlastStation2.  BlastStation-Free has all features of BlastStation2, such as NCBI-Blast search using the EBI server,  WU-Blast2 search using the EBI server, and Standalone BLAST search on your PC with some limitations.
 

Major features

• EBI NCBI-Blast search via web service^.
• EBI WU-Blast2 search via web service.
• Local BLAST search with 32-bit NCBI BLAST 2.2.25^*) on your PC.
• Easy database download from NCBI ftp server to your PC.
• Easy database creation from your FASTA or FASTQ file, which can be compressed in .gz, .Z, or .zip format.^*)
• Easy management of BLAST search parameters, FASTA data and search results.
• Graphical display of search results.
• Summary table display of search results.
• Export summary table in CSV format.
• Export search results sequences in FASTA format.

Limitations

• Up to 5^*) FASTA data submission in one BlastStation job.
• Available programs are blastn, blastp, and blastx.^*)
• Local database input file size is limited to 25 MB, either compressed or not compressed.^*)
• One core is used for local blast search.^*)
• Function to change database folder is disabled.
• Function to display alignment detail is disabled.^*)
• No support.
• Use behaviors will be collected and will be used for future improvements.

System Requirements

• Mac OS X 10.4.11, 10.5.8, 10.6.8, 10.7^*) or later
• PowerPC or Intel processor
• 512 MB RAM

^*) BlastStation-Free ver.2 or later